| Team Name | City | State/Prov | Status | Reg/Wait List # |
| Force Softball | Westport | MA | Accepted (Pending USA Reg) | |
| GSE 16U Wessling | Epsom | NH | Accepted (Pending USA Reg) | |
| Impact South | PORTSMOUTH | RI | Accepted (Pending USA Reg) | |
| Khaos 16u Robbins | Paris | ME | Accepted (Pending USA Reg) | |
| Lady Sting Martins | Middleboro | MA | Accepted (Pending USA Reg) | 750 |
| Miss Shen Revolution | Clifton Park | NY | Accepted (Pending USA Reg) | |
| NE IMPACT | Pawtucket | RI | Accepted (Pending USA Reg) | 750 |
| RI Stonecrabs 16U | Warwick | RI | Accepted (Pending USA Reg) | |
| South Troy Dodgers | Troy | NY | Accepted (Pending USA Reg) | 750 |
| Taunton Tornadoes | Taunton | MA | Accepted (Pending USA Reg) | 750 |
| Warriors Softball Club | Andover | MA | Accepted (Pending USA Reg) | |
| Worcester Union 16U Ryzewski | Worcester | MA | Withdrawn | |